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Catalog Record: Feasibility of using a particle counter or flow-cytometer for bacterial enumeration in the assimilable organic carbon (AOC) analysis method
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Organizations

EPSCoR-ACE
Primary

Funding
NSF

Feasibility of using a particle counter or flow-cytometer for bacterial enumeration in the assimilable organic carbon (AOC) analysis method

Assimilable organic carbon (AOC) is one of the major determinants of microbial growth and stability in drinking water distribution systems. Nevertheless, AOC measurements are rarely conducted in practice owing, in part, to the tedious and time-consuming nature of the bioassay. Herein, we compared three alternative cell count approaches [flow cytometry with staining (FC-S), flow cytometry without staining (FC-NS), and particle counting (Coulter counter; CC)] for bacterial enumeration as a means to expedite the AOC bioassay. Our results suggest that of the three methods only FC-S provides a suitable alternative to plate counting for rapid and accurate enumeration of both P17 and NOX in the AOC bioassay. While the cell counts obtained by FC-NS were linearly correlated with those obtained using the traditional heterotrophic plate count (HPC) method (FC-NS: R2 = 0.89–0.96), the AOC values obtained by FC-NS were overestimated by 18–57 %. The CC approach was unsuccessful in enumerating Spirillum strain NOX cells because of the relatively small size of that organism. The CC counts were linearly correlated with HPC for Pseudomonas fluorescens strain P-17 (P17) cells (R2 = 0.83) but like FC-NS, the CC approach also overestimated the AOC values (for P-17). The advantage of the FC-S method over the other two is improved sensitivity and the ability to specifically enumerate whole cells (and likely viable) as opposed to non-viable cells, cell debris, and other contaminating particles introduced by the test water itself or sample handling.

Complete

Data and Resources

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    Website :: Publisher's website

    http://link.springer.com/article/10.1007/s10532-015-9741-6

Status: Complete
Type: Project
Data Types: Report
Primary Contacts
Name: Tania Clucas
Email: thclucas@alaska.edu
Primary Organizations
Type
EPSCoR - Alaska Adapting to Changing Environments
Academic

Funding Organization
Type
EPSCoR - Alaska Adapting to Changing Environments
Academic

Other Organizations
National Science Foundation

Regions
Alaska

Direct Record Link
http://northern.epscor.alaska.edu/catalog/entries/8859-feasibility-of-using-a-particle-counter-or-flo
Direct Full Map Link
http://northern.epscor.alaska.edu/catalog/entries/8859-feasibility-of-using-a-particle-counter-or-flo/map